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Identification of morphological and biochemical changes in keratin‐8/18 knock‐down cells using Raman spectroscopy
Author(s) -
Singh S. P.,
Alam Hunain,
Dmello Crismita,
Mamgain Hitesh,
Vaidya Milind M.,
Dasari Ramachandra Rao,
Krishna C. Murali
Publication year - 2017
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201600249
Subject(s) - raman spectroscopy , fiber , materials science , biomedical engineering , nanotechnology , computational biology , biophysics , medicine , biology , optics , physics , composite material
Accurate understanding of cellular processes and responses to stimuli is of paramount importance in biomedical research and diagnosis. Raman spectroscopy (RS), a label‐free and nondestructive spectroscopic method has the potential to serve as a novel ’theranostics' tool. Both fiber‐optic and micro‐Raman studies have demonstrated efficacy in diagnostics and therapeutic response monitoring. In the present study, we have evaluated the potential of micro‐Raman spectroscopic maps in identifying changes induced by loss of K8/18 proteins in a tongue cancer cell line. Furthermore, we also evaluated the efficacy of less expensive and commercially available fiber probes to identify K8/18 wild and knock‐down cell pellets, in view of the utility of cell pellet‐based studies. The findings suggest that major differences in the cellular morphology and biochemical composition can be objectively identified and can be utilized for classification using both micro‐Raman and fiber‐probe‐based RS. These findings highlight the potential of fiber‐optic probe‐based RS in noninvasive cellular phenotyping for diagnosis and therapeutic response monitoring, especially in low‐resource settings.