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Distinction between breast cancer cell subtypes using third harmonic generation microscopy
Author(s) -
Gavgiotaki Evangelia,
Filippidis George,
Markomanolaki Haris,
Kenanakis George,
Agelaki Sofia,
Georgoulias Vassilis,
Athanassakis Irene
Publication year - 2017
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201600173
Subject(s) - microscopy , lipid raft , lipid bilayer , cancer cell , cancer , fourier transform infrared spectroscopy , breast cancer , cell , biophysics , materials science , chemistry , nuclear magnetic resonance , pathology , membrane , medicine , biology , optics , biochemistry , physics
Third Harmonic Generation (THG) microscopy as a non‐invasive, label free imaging methodology, allows linkage of lipid profiles with various breast cancer cells. The collected THG signal arise mostly from the lipid droplets and the membrane lipid bilayer. Quantification of THG signal can accurately distinguish HER2‐positive cells. Further analysis using Fourier transform infrared (FTIR) spectra reveals cancer‐specific profiles, correlating lipid raft‐corresponding spectra to THG signal, associating thus THG to chemical information.THG imaging of a cancer cell.