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Direct and label‐free detection of the human growth hormone in urine by an ultrasensitive bimodal waveguide biosensor
Author(s) -
GonzálezGuerrero Ana Belén,
Maldonado Jesús,
Dante Stefania,
Grajales Daniel,
Lechuga Laura M.
Publication year - 2017
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201600154
Subject(s) - biosensor , detection limit , transducer , urine , chromatography , homogeneous , matrix (chemical analysis) , chemistry , interferometry , materials science , resolution (logic) , optics , nanotechnology , acoustics , computer science , biochemistry , physics , artificial intelligence , thermodynamics
A label‐free interferometric transducer showing a theoretical detection limit for homogeneous sensing of 5 × 10 –8 RIU, being equivalent to a protein mass coverage resolution of 2.8 fg mm –2 , is used to develop a high sensitive biosensor for protein detection. The extreme sensitivity of this transducer combined with a selective bioreceptor layer enables the direct evaluation of the human growth hormone (hGH) in undiluted urine matrix in the 10 pg mL –1 range.