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A compact fiber‐optic probe‐based singlet oxygen luminescence detection system
Author(s) -
Gemmell Nathan R.,
McCarthy Aongus,
Kim Michele M.,
Veilleux Israel,
Zhu Timothy C.,
Buller Gerald S.,
Wilson Brian C.,
Hadfield Robert H.
Publication year - 2017
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201600078
Subject(s) - luminescence , photosensitizer , singlet oxygen , optoelectronics , materials science , detector , optics , photon counting , avalanche photodiode , diode , photon , chemistry , oxygen , physics , photochemistry , organic chemistry
This paper presents a novel compact fiberoptic based singlet oxygen near‐infrared luminescence probe coupled to an InGaAs/InP single photon avalanche diode (SPAD) detector. Patterned time gating of the single‐photon detector is used to limit unwanted dark counts and eliminate the strong photosensitizer luminescence background. Singlet oxygen luminescence detection at 1270 nm is confirmed through spectral filtering and lifetime fitting for Rose Bengal in water, and Photofrin in methanol as model photosensitizers. The overall performance, measured by the signal‐to‐noise ratio, improves by a factor of 50 over a previous system that used a fiberoptic‐coupled superconducting nanowire single‐photon detector. The effect of adding light scattering to the photosensitizer is also examined as a first step towards applications in tissue in vivo .