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Minimally invasive screening for colitis using attenuated total internal reflectance fourier transform infrared spectroscopy
Author(s) -
Titus Jitto,
Viennois Emilie,
Merlin Didier,
Unil Perera A. G.
Publication year - 2017
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201600041
Subject(s) - attenuated total reflection , colitis , ulcerative colitis , inflammatory bowel disease , arthritis , fourier transform infrared spectroscopy , in vivo , medicine , mannose , chemistry , infrared spectroscopy , immunology , biology , biochemistry , disease , optics , physics , microbiology and biotechnology , organic chemistry
This article describes a rapid, simple and cost‐effective technique that could lead to a screening method for colitis without the need for biopsies or in vivo measurements. This screening technique includes the testing of serum using Attenuated Total Reflectance Fourier Transform Infrared (ATR‐FTIR) spectroscopy for the colitis‐induced increased presence of mannose. Chronic (Interleukin 10 knockout) and acute (Dextran Sodium Sulphate‐induced) models for colitis are tested using the ATR‐FTIR technique. Arthritis (Collagen Antibody Induced Arthritis) and metabolic syndrome (Toll like receptor 5 knockout) models are also tested as controls. The marker identified as mannose uniquely screens and distinguishes the colitic from the non‐colitic samples and the controls. The reference or the baseline spectrum could be the pooled and averaged spectra of non‐colitic samples or the subject's previous sample spectrum. This shows the potential of having individualized route maps of disease status, leading to personalized diagnosis and drug management.