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Low‐level laser therapy stimulates the oxidative burst in human neutrophils and increases their fungicidal capacity
Author(s) -
Cerdeira Cláudio Daniel,
Lima Brigagão Maísa Ribeiro Pereira,
Carli Marina Lara,
Souza Ferreira Cláudia,
Oliveira Isac Moraes Gabriel,
Hadad Henrique,
Costa Hanemann João Adolfo,
Hamblin Michael R.,
Sperandio Felipe Fornias
Publication year - 2016
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201600035
Subject(s) - respiratory burst , reactive oxygen species , candida albicans , superoxide , chemistry , hypochlorite , radical , in vitro , oxidative phosphorylation , microbiology and biotechnology , photochemistry , biochemistry , biology , enzyme , organic chemistry
Low‐level laser therapy (LLLT) is known to enhance mitochondrial electron transfer and ATP production; thus, this study asked whether LLLT could stimulate the oxidative burst in human neutrophils (PMN) and improve their ability to kill microorganisms. Blood from healthy human subjects was collected and PMN were isolated from the samples. PMN were treated in vitro with 660 nm or 780 nm CW laser light at 40 mW power and increasing energies up to 19.2 J and were subsequently incubated with Candida albicans cells. Generation of hydroxyl radicals, hypochlorite anions and superoxide anions by PMN were checked using fluorescent probes and chemiluminescence assays; a microbicidal activity assay against C. albicans was also performed. LLLT excited PMN to a higher functional profile, which was translated as superior production of reactive oxygen species (ROS) and increased fungicidal capacity. The most efficacious energy was 19.2 J and, interestingly, the 660 nm light was even more efficacious than 780 nm at increasing the respiratory burst of PMN and the fungicidal capacity.Human neutrophils (PMN) were stimulated in vitro with 660 nm or 780 nm CW laser light at 40 mW of power and a total energy of 19.2 J. Low‐level laser therapy (LLLT) excited PMN to a higher functional profile, which was translated as a superior production of reactive oxygen species (ROS) such as hydroxyl radicals (HO • ) and hypochlorite anions (ClO − ) (Figure) and increased fungicidal capacity against Candida albicans cells.