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Back Cover: Quantifying nanoscale biochemical heterogeneity in human epithelial cancer cells using combined AFM and PTIR absorption nanoimaging (J. Biophotonics 1–2/2015)
Publication year - 2015
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201570011
Subject(s) - biophotonics , nanoscopic scale , absorption (acoustics) , cover (algebra) , nanotechnology , photothermal therapy , biophysics , chemistry , optics , materials science , physics , biology , photonics , mechanical engineering , engineering
The configuration of interacting subcellular chemical structures governs much of the biomechanics underlying cell structure‐function relationships. To date, rapid and non‐destructive measurement of subcellular chemical heterogeneity on the nanoscale have proved elusive. In this issue, Kennedy et al. describe how iterative measurement of the variation in density normalized chemical absorption based on photothermal induced resonance, in conjunction with morphological image processing permits several unique imaging properties for human epithelial cells including: sub‐50 nm resolution chemically resolved membrane boundaries, label free localization of nuclei and quantitative measurement of chemical heterogeneity from sub‐50 nm point sources. The cover image illustrates topographic and nanoscale infrared imaging of 4 colon cancer cells with insets of 4 recovered high resolution chemical complexity maps. (Picture: E. Kennedy et al., pp. 133–141 in this issue)