Premium
Front Cover: Quantifying molecular colocalization in live cell fluorescence microscopy (J. Biophotonics 1–2/2015)
Publication year - 2015
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201570010
Subject(s) - colocalization , front cover , biophotonics , fluorescence microscope , microscopy , fluorescence , physics , biology , cover (algebra) , optics , microbiology and biotechnology , photonics , mechanical engineering , engineering
A novel and quantitative method for determining the degree of colocalization in live‐cell fluorescence microscopy images for two and more data channels is presented by F. Humpert et al. The composite image resembles assets in Gamma‐norm based colocalization studies. In detail: (left) 3‐channel colocalization is measured on a multichannel test image that shows colocalizing spot patterns, (top) an overlaying topology map highlighting colocalized regions in the live cell fluorescence microscopy image beneath, (background) fluorescently co‐labeled tubulin and actin structures in Cos‐7 cells. (Picture: F. Humpert et al., pp. 124–132 in this issue)