Premium
Nanodiamond for biolabelling and toxicity evaluation in the zebrafish embryo in vivo
Author(s) -
Lin Y.C.,
Wu K.T.,
Lin Z.R.,
Perevedentseva E.,
Karmenyan A.,
Lin M.D.,
Cheng C.L.
Publication year - 2016
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201500304
Subject(s) - nanodiamond , zebrafish , nanotechnology , in vivo , nanotoxicology , biocompatibility , materials science , biomedical engineering , chemistry , biology , nanoparticle , microbiology and biotechnology , medicine , biochemistry , diamond , metallurgy , composite material , gene
Nanodiamond (ND) has been proposed for various biomedical applications, including bioimaging, biosensing and drug delivery, owing to its physical‐chemical properties and biocompatibility. Particularly, ND has been demonstrated as fluorescence‐ and Raman‐detectable labels in many cellular models. Different surface functionalization methods have been developed, varying the ND's surface properties and rendering the possibility to attach biomolecules to provide interaction with biological targets. For this, toxicity is of major concern in animal models. Aside from cellular models, a cost‐effective animal test will greatly facilitate the development of applications. In this study, we use the rapid, sensitive and reproducible zebrafish embryo model for in vivo nanotoxicity test. We optimize the conditions for using this animal model and analyze the zebrafish embryonic development in the presence of ND. ND is observed in the embryo in vivo using laser confocal fluorescence microscopy and fluorescence lifetime imaging. Using the zebrafish model for a safety evaluation of ND‐based nanolabel is discussed.