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Development and in vivo testing of a high frequency endoscopic Raman spectroscopy system for potential applications in the detection of early colonic neoplasia
Author(s) -
Short Michael A.,
Wang Wenbo,
Tai Isabella T.,
Zeng Haishan
Publication year - 2016
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201500205
Subject(s) - in vivo , raman spectroscopy , pathology , colonoscopy , biomedical engineering , medicine , colorectal cancer , cancer , optics , biology , physics , microbiology and biotechnology
The objective of this study was to build and test an adjunct system to a colonoscope for in vivo measurement of Raman spectra from colon tissue for potentially improving the detection of early cancers. The novelty of this system was that low cost fibre optic probes were used, without the addition of expensive optical filters. Good quality in vivo Raman spectra were successfully obtained with a 1 s integration time in the high frequency (HF) range from normal tissue and polyps of patients during a colonoscopy. The polyps were subsequently removed, and their pathology determined. The acquired in vivo Raman spectra showed clear changes between tissue with normal and tubular adenoma pathology. Further clinical study with this low cost HF Raman probe is warranted to fully test its clinical utility.Left: Raman probe orientated on a suspected polyp (indicated by arrow) under video surveillance during a colonoscopy. Right: average Raman spectra from 2800–3050 cm –1 obtained from polyps at different stages of disease. The peak intensities are in arbitrary units.