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Automated quantitative cytological analysis using portable microfluidic microscopy
Author(s) -
Jagannadh Veerendra Kalyan,
Murthy Rashmi Sreeramachandra,
Srinivasan Rajesh,
Gorthi Sai Siva
Publication year - 2016
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201500108
Subject(s) - microfluidics , computer science , microscopy , sample (material) , automation , biomedical engineering , sample preparation , artificial intelligence , biological system , nanotechnology , pathology , biology , materials science , chromatography , chemistry , engineering , medicine , mechanical engineering
In this article, a portable microfluidic microscopy based approach for automated cytological investigations is presented. Inexpensive optical and electronic components have been used to construct a simple microfluidic microscopy system. In contrast to the conventional slide‐based methods, the presented method employs microfluidics to enable automated sample handling and image acquisition. The approach involves the use of simple in‐suspension staining and automated image acquisition to enable quantitative cytological analysis of samples. The applicability of the presented approach to research in cellular biology is shown by performing an automated cell viability assessment on a given population of yeast cells. Further, the relevance of the presented approach to clinical diagnosis and prognosis has been demonstrated by performing detection and differential assessment of malaria infection in a given sample.