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Light distribution and thermal effects in the rat brain under optogenetic stimulation
Author(s) -
Gysbrechts Barbara,
Wang Ling,
Trong Nghia Nguyen Do,
Cabral Henrique,
Navratilova Zaneta,
Battaglia Francesco,
Saeys Wouter,
Bartic Carmen
Publication year - 2016
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201500106
Subject(s) - optogenetics , thalamus , neuroscience , stimulation , monte carlo method , striatum , deep brain stimulation , optics , materials science , biophysics , biology , physics , medicine , mathematics , statistics , dopamine , disease , pathology , parkinson's disease
Optical brain stimulation gained a lot of attention in neuroscience due to its superior cell‐type specificity. In the design of illumination strategies, predicting the light propagation in a specific tissue is essential and requires knowledge of the optical properties of that tissue. We present the estimated absorption and reduced scattering in rodent brain tissue using non‐destructive contact spatially resolved spectroscopy (cSRS). The obtained absorption and scattering in the cortex, hippocampus and striatum are similar, but lower than in the thalamus, leading to a less deep but broader light penetration profile in the thalamus. Next, the light distribution was investigated for different stimulation protocols relevant for fiber‐optic based optogenetic experiments, using Monte Carlo simulation. A protocol specific analysis is proposed to evaluate the potential of thermally induced side effects.