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Label‐free in vivo imaging of peripheral nerve by multispectral photoacoustic tomography
Author(s) -
Li Rui,
Phillips Evan,
Wang Pu,
Goergen Craig J.,
Cheng JiXin
Publication year - 2016
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201500004
Subject(s) - multispectral image , tomography , femoral artery , biomedical engineering , image resolution , medicine , radiology , geology , artificial intelligence , remote sensing , computer science
Unintentional surgical damage to nerves is mainly due to poor visualization of nerve tissue relative to adjacent structures. Multispectral photoacoustic tomography can provide chemical information with specificity and ultrasonic spatial resolution with centimeter imaging depth, making it a potential tool for noninvasive neural imaging. To implement this label‐free imaging approach, a multispectral photoacoustic tomography platform was built. Imaging depth and spatial resolution were characterized. In vivo imaging of the femoral nerve that is 2 mm deep in a nude mouse was performed. Through multivariate curve resolution analysis, the femoral nerve was discriminated from the femoral artery and chemical maps of their spatial distributions were generated.The femoral nerve was discriminated from the femoral artery by multivariate curve resolution analysis.