z-logo
Premium
Differential remodeling of extracellular matrices by breast cancer initiating cells
Author(s) -
Raja Anju M.,
Xu Shuoyu,
Zhuo Shuangmu,
Tai Dean C.S.,
Sun Wanxin,
So Peter T.C.,
Welsch Roy E.,
Chen ChienShing,
Yu Hanry
Publication year - 2015
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201400079
Subject(s) - extracellular matrix , cancer research , cancer , cancer cell , extracellular , breast cancer , in vitro , chemistry , medicine , biology , pathology , microbiology and biotechnology , biochemistry
Cancer initiating cells (CICs) have been the focus of recent anti‐cancer therapies, exhibiting strong invasion capability via potentially enhanced ability to remodel extracellular matrices (ECM). We have identified CICs in a human breast cancer cell line, MX‐1, and developed a xenograft model in SCID mice. We investigated the CICs' matrix‐remodeling effects using Second Harmonic Generation (SHG) microscopy to identify potential phenotypic signatures of the CIC‐rich tumors. The isolated CICs exhibit higher proliferation, drug efflux and drug resistant properties in vitro ; were more tumorigenic than non‐CICs, resulting in more and larger tumors in the xenograft model. The CIC‐rich tumors have less collagen in the tumor interior than in the CIC‐poor tumors supporting the idea that the CICs can remodel the collagen more effectively. The collagen fibers were preferentially aligned perpendicular to the CIC‐rich tumor boundary while parallel to the CIC‐poor tumor boundary suggesting more invasive behavior of the CIC‐rich tumors. These findings would provide potential translational values in quantifying and monitoring CIC‐rich tumors in future anti‐cancer therapies.CIC‐rich tumors remodel the collagen matrix more than CIC‐poor tumors.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here