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Superpulsed (Ga‐As, 904 nm) low‐level laser therapy (LLLT) attenuates inflammatory response and enhances healing of burn wounds
Author(s) -
Gupta Asheesh,
Keshri Gaurav K.,
Yadav Anju,
Gola Shefali,
Chauhan Satish,
Salhan Ashok K.,
Bala Singh Shashi
Publication year - 2015
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201400058
Subject(s) - low level laser therapy , silver sulfadiazine , hydroxyproline , wound healing , burn wound , inflammation , matrix metalloproteinase , medicine , sulfadiazine , angiogenesis , laser , inflammatory response , laser therapy , chemistry , surgery , biochemistry , physics , optics , antibiotics
Low‐level laser therapy (LLLT) using superpulsed near‐infrared light can penetrate deeper in the injured tissue and could allow non‐pharmacological treatment for chronic wound healing. This study investigated the effects of superpulsed laser (Ga‐As 904 nm, 200 ns pulse width; 100 Hz; 0.7 mW mean output power; 0.4 mW/cm 2 average irradiance; 0.2 J/cm 2 total fluence) on the healing of burn wounds in rats, and further explored the probable associated mechanisms of action. Irradiated group exhibited enhanced DNA, total protein, hydroxyproline and hexosamine contents compared to the control and silver sulfadiazine (reference care) treated groups. LLLT exhibited decreased TNF‐α level and NF‐ k B, and up‐regulated protein levels of VEGF, FGFR‐1, HSP‐60, HSP‐90, HIF‐1α and matrix metalloproteinases‐2 and 9 compared to the controls. In conclusion, LLLT using superpulsed 904 nm laser reduced the inflammatory response and was able to enhance cellular proliferation, collagen deposition and wound contraction in the repair process of burn wounds.Photomicrographs showing no, absence inflammation and faster wound contraction in LLLT superpulsed (904 nm) laser treated burn wounds as compared to the non‐irradiated control and silver sulfadiazine (SSD) ointment (reference care) treated wounds