Premium
Three‐dimensional arbitrary trajectory scanning photoacoustic microscopy
Author(s) -
Yeh Chenghung,
Soetikno Brian,
Hu Song,
Maslov Konstantin I.,
Wang Lihong V.
Publication year - 2015
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201400055
Subject(s) - raster scan , schematic , materials science , microscopy , scanning electron microscope , optics , biomedical engineering , physics , medicine , electronic engineering , engineering
We have enhanced photoacoustic microscopy with three‐dimensional arbitrary trajectory (3‐DAT) scanning, which can rapidly image selected vessels over a large field of view (FOV) and maintain a high signal‐to‐noise ratio (SNR) despite the depth variation of the vessels. We showed that hemoglobin oxygen saturation (sO 2 ) and blood flow can be measured simultaneously in a mouse ear in vivo at a frame rate 67 times greater than that of a traditional two‐dimensional raster scan. We also observed sO 2 dynamics in response to switching from systemic hypoxia to hyperoxia.3‐DAT‐scanning photoacoustic microscopy. Schematic diagram of the 3D scanning stage and method.