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The use of optical trap and microbeam to investigate the mechanical and transport characteristics of tunneling nanotubes in tumor spheroids
Author(s) -
Patheja Pooja,
Dasgupta Raktim,
Dube Alok,
Ahlawat Sunita,
Verma Ravi Shanker,
Gupta Pradeep Kumar
Publication year - 2015
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201400039
Subject(s) - optical tweezers , microbeam , spheroid , tweezers , materials science , fluorescence , nanotechnology , tumor cells , vesicle , biophysics , optoelectronics , chemistry , optics , membrane , physics , in vitro , biology , biochemistry , cancer research
The use of optical trap and microbeam for investigating mechanical and transport properties of inter cellular tunneling nanotubes (TnTs) in tumor spheroids has been demonstrated. TnTs in tumor spheroids have been visualized by manipulating TnT connected cells using optical tweezers. Functionality of the TnTs for transferring cytoplasmic vesicles and injected dye molecules by optoporation method has been studied. Further, the TnTs could be longitudinally stretched by manipulating the connected cells and their elastic response was studied.Manipulation of cells at the surface of tumor spheroid using optical tweezers and injection of fluorescent dye into a trapped cell using optoporation technique.