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Wide‐field hyperspectral 3D imaging of functionalized gold nanoparticles targeting cancer cells by reflected light microscopy
Author(s) -
Sergiy Patskovsky,
Éric Bergeron,
David Rooney,
Michel Meunier
Publication year - 2015
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201400025
Subject(s) - hyperspectral imaging , supercontinuum , microscopy , colloidal gold , spectral signature , dark field microscopy , materials science , spectral imaging , optics , nanotechnology , computer science , nanoparticle , artificial intelligence , optoelectronics , remote sensing , physics , wavelength , photonic crystal fiber , geology
We present a new hyperspectral reflected light microscopy system with a scanned broadband supercontinuum light source. This wide‐field and low phototoxic hyperspectral imaging system has been successful for performing spectral three‐dimensional (3D) localization and spectroscopic identification of CD44‐targeted PEGylated AuNPs in fixed cell preparations. Such spatial and spectral information is essential for the improvement of nanoplasmonic‐based imaging, disease detection and treatment in complex biological environment. The presented system can be used for real‐time 3D NP tracking as spectral sensors, thus providing new avenues in the spatio‐temporal characterization and detection of bioanalytes.3D image of the distribution of functionalized AuNPs attached to CD44‐expressing MDA‐MB‐231 human cancer cells.