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Dual‐polarization Raman spectral imaging to extract overlapping molecular fingerprints of living cells
Author(s) -
Chiu Liangda,
Palonpon Almar F.,
Smith Nicholas I.,
Kawata Satoshi,
Sodeoka Mikiko,
Fujita Katsumasa
Publication year - 2015
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201300204
Subject(s) - raman spectroscopy , hyperspectral imaging , chemical imaging , polarization (electrochemistry) , spectral imaging , hela , materials science , raman microspectroscopy , optics , optoelectronics , artificial intelligence , chemistry , computer science , physics , cell , biochemistry
Raman spectral imaging is gaining more and more attention in biological studies because of its label‐free characteristic. However, the discrimination of overlapping chemical contrasts has been a major challenge. In this study, we introduce an optical method to simultaneously obtain two orthogonally polarized Raman images from a single scan of the sample. We demonstrate how this technique can improve the quality and quantity of the hyperspectral Raman dataset and how the technique is expected to further extend the horizons of Raman spectral imaging in biological studies by providing more detailed chemical information.The dual‐polarization Raman images of a HeLa cell.