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A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue
Author(s) -
Sparks Hugh,
Warren Sean,
Guedes Joana,
Yoshida Nagisa,
Charn Tze Choong,
Guerra Nadia,
Tatla Taranjit,
Dunsby Christopher,
French Paul
Publication year - 2015
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201300203
Subject(s) - autofluorescence , fluorescence lifetime imaging microscopy , excitation , endoscope , materials science , laser , ex vivo , fluorescence , biomedical engineering , optics , in vivo , optoelectronics , physics , medicine , biology , quantum mechanics , radiology , microbiology and biotechnology
Fluorescence lifetime imaging (FLIM) has previously been shown to provide contrast between normal and diseased tissue. Here we present progress towards clinical and preclinical FLIM endoscopy of tissue autofluorescence, demonstrating a flexible wide‐field endoscope that utilised a low average power blue picosecond laser diode excitation source and was able to acquire ∼mm‐scale spatial maps of autofluorescence lifetimes from fresh ex vivo diseased human larynx biopsies in ∼8 seconds using an average excitation power of ∼0.5 mW at the specimen. To illustrate its potential for FLIM at higher acquisition rates, a higher power mode‐locked frequency doubled Ti:Sapphire laser was used to demonstrate FLIM of ex vivo mouse bowel at up to 2.5 Hz using 10 mW of average excitation power at the specimen. (© 2014 WILEY‐VCH Verlag GmbH &Co. KGaA, Weinheim)