Live cell imaging of the intracellular compartmentalization of the contaminate benzo[a]pyrene | Zendy

Ali Rizwan | Zendy; Trump Saskia | Zendy; Lehmann Irina | Zendy; Hanke Thomas | Zendy

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Live cell imaging of the intracellular compartmentalization of the contaminate benzo[a]pyrene

Author(s) -

Ali Rizwan,

Trump Saskia,

Lehmann Irina,

Hanke Thomas

Publication year - 2015

Publication title -

journal of biophotonics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.877

H-Index - 66

eISSN - 1864-0648

pISSN - 1864-063X

DOI - 10.1002/jbio.201300170

Subject(s) - pyrene , intracellular , compartmentalization (fire protection) , chemistry , benzo(a)pyrene , endoplasmic reticulum , confocal microscopy , bleb (medicine) , confocal , lipid droplet , biophysics , fluorescence , fluorescence microscope , flow cytometry , fluorescence lifetime imaging microscopy , microbiology and biotechnology , cell , biochemistry , biology , organic chemistry , physics , trabeculectomy , geometry , mathematics , quantum mechanics , neuroscience , glaucoma , enzyme

This study investigates the cellular response of murine hepatoma cells to the polycyclic aromatic hydrocarbon benzo[a]pyrene (B[a]P) using two‐photon and confocal laser scanning microscopy. The intracellular distribution of B[a]P and the B[a]P/AhR complex was visualized time‐ and concentration‐dependent for up to 48 h of exposure. B[a]P was predominantly found in lipid droplets, endoplasmic reticulum and lysosomes, where B[a]P is collected and forms large aggregates. Changes in mitochondrial membrane potential and bleb formation due to high B[a]P concentrations were observed. The imaging data presented in this study provide new insights into the systemic cellular regulation following B[a]P exposure. (© 2014 WILEY‐VCH Verlag GmbH &Co. KGaA, Weinheim)

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