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Live cell imaging of the intracellular compartmentalization of the contaminate benzo[a]pyrene
Author(s) -
Ali Rizwan,
Trump Saskia,
Lehmann Irina,
Hanke Thomas
Publication year - 2015
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201300170
Subject(s) - pyrene , intracellular , compartmentalization (fire protection) , chemistry , benzo(a)pyrene , endoplasmic reticulum , confocal microscopy , bleb (medicine) , confocal , lipid droplet , biophysics , fluorescence , fluorescence microscope , flow cytometry , fluorescence lifetime imaging microscopy , microbiology and biotechnology , cell , biochemistry , biology , organic chemistry , physics , trabeculectomy , geometry , mathematics , quantum mechanics , neuroscience , glaucoma , enzyme
This study investigates the cellular response of murine hepatoma cells to the polycyclic aromatic hydrocarbon benzo[a]pyrene (B[a]P) using two‐photon and confocal laser scanning microscopy. The intracellular distribution of B[a]P and the B[a]P/AhR complex was visualized time‐ and concentration‐dependent for up to 48 h of exposure. B[a]P was predominantly found in lipid droplets, endoplasmic reticulum and lysosomes, where B[a]P is collected and forms large aggregates. Changes in mitochondrial membrane potential and bleb formation due to high B[a]P concentrations were observed. The imaging data presented in this study provide new insights into the systemic cellular regulation following B[a]P exposure. (© 2014 WILEY‐VCH Verlag GmbH &Co. KGaA, Weinheim)