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It's in your blood: spectral biomarker candidates for urinary bladder cancer from automated FTIR spectroscopy
Author(s) -
Ollesch Julian,
Heinze Margot,
Heise H. Michael,
Behrens Thomas,
Brüning Thomas,
Gerwert Klaus
Publication year - 2014
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201300163
Subject(s) - absorbance , fourier transform infrared spectroscopy , urinary system , spectroscopy , bladder cancer , analytical chemistry (journal) , chemistry , chromatography , biomedical engineering , materials science , medicine , cancer , optics , physics , quantum mechanics
Blood samples of urinary bladder cancer (UBC) patients and patients with urinary tract infection were analysed with advanced automated high throughput Fourier transform infrared (HT‐FTIR)‐spectroscopy. Thin dried film samples were robotically prepared on multi‐well titer plates (MTP) for absorbance measurements in transmission mode. Within the absorbance, 1st and 2nd derivative spectra of serum and two plasma preparations, discriminative patterns were identified and validated using bioinformatic tools. The optimal spectral resolution for data acquisition was determined. An accurate discrimination of the patient groups was achieved with three different independent spectral variable sets. The HT‐FTIR blood test may support future clinical diagnostics. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)