Quantitative measurement of blood velocity in zebrafish with optical vector field tomography | Zendy

Fieramonti Luca | Zendy; Foglia Efrem A. | Zendy; Malavasi Stefano | Zendy; D'Andrea Cosimo | Zendy; Valentini Gianluca | Zendy; Cotelli Franco | Zendy; Bassi Andrea | Zendy

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Quantitative measurement of blood velocity in zebrafish with optical vector field tomography

Author(s) -

Fieramonti Luca,

Foglia Efrem A.,

Malavasi Stefano,

D'Andrea Cosimo,

Valentini Gianluca,

Cotelli Franco,

Bassi Andrea

Publication year - 2015

Publication title -

journal of biophotonics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.877

H-Index - 66

eISSN - 1864-0648

pISSN - 1864-063X

DOI - 10.1002/jbio.201300162

Subject(s) - zebrafish , vector field , blood flow , tomography , biomedical engineering , flow velocity , biological system , computer science , computer vision , biology , artificial intelligence , optics , flow (mathematics) , physics , radiology , medicine , mechanics , biochemistry , gene

Microscopy techniques can readily visualize the finest details of embryo vasculature, but still lack to provide a complete three‐dimensional representation of blood flow parameters. We present an in‐vivo 3D imaging technique, able to reconstruct the blood cell velocity vector over a large volume of zebrafish embryos. This low cost and relatively simple technique is exploited to quantitatively assess blood velocity in the zebrafish tail at different stages of development. (© 2013 WILEY‐VCH Verlag GmbH &Co. KGaA, Weinheim)

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