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Quantitative measurement of blood velocity in zebrafish with optical vector field tomography
Author(s) -
Fieramonti Luca,
Foglia Efrem A.,
Malavasi Stefano,
D'Andrea Cosimo,
Valentini Gianluca,
Cotelli Franco,
Bassi Andrea
Publication year - 2015
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201300162
Subject(s) - zebrafish , vector field , blood flow , tomography , biomedical engineering , flow velocity , biological system , computer science , computer vision , biology , artificial intelligence , optics , flow (mathematics) , physics , radiology , medicine , mechanics , biochemistry , gene
Microscopy techniques can readily visualize the finest details of embryo vasculature, but still lack to provide a complete three‐dimensional representation of blood flow parameters. We present an in‐vivo 3D imaging technique, able to reconstruct the blood cell velocity vector over a large volume of zebrafish embryos. This low cost and relatively simple technique is exploited to quantitatively assess blood velocity in the zebrafish tail at different stages of development. (© 2013 WILEY‐VCH Verlag GmbH &Co. KGaA, Weinheim)