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Vibrational biospectroscopy coupled with multivariate analysis extracts potentially diagnostic features in blood plasma/serum of ovarian cancer patients
Author(s) -
Owens Gemma L.,
Gajjar Ketan,
Trevisan Júlio,
Fogarty Simon W.,
Taylor Siân E.,
Da GamaRose Bianca,
MartinHirsch Pierre L.,
Martin Francis L.
Publication year - 2014
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201300157
Subject(s) - ovarian cancer , raman spectroscopy , principal component analysis , medicine , blood plasma , cancer , gastroenterology , chemistry , artificial intelligence , physics , computer science , optics
Despite numerous advances in “omics” research, early detection of ovarian cancer still remains a challenge. The aim of this study was to determine whether attenuated total reflection Fourier‐transform infrared (ATR‐FTIR) or Raman spectroscopy could characterise alterations in the biomolecular signatures of human blood plasma/serum obtained from ovarian cancer patients compared to non‐cancer controls. Blood samples isolated from ovarian cancer patients ( n = 30) and healthy controls ( n = 30) were analysed using ATR‐FTIR spectroscopy. For comparison, a smaller cohort of samples ( n = 8) were analysed using an InVia Renishaw Raman spectrometer. Resultant spectra were pre‐processed prior to being inputted into principal component analysis (PCA) and linear discriminant analysis (LDA). Statistically significant differences ( P < 0.001) were observed between spectra of ovarian cancer versus control subjects for both biospectroscopy methods. Using a support vector machine classifier for Raman spectra of blood plasma, a diagnostic accuracy of 74% was achieved, while the same classifier showed 93.3% accuracy for IR spectra of blood plasma. These observations suggest that a biospectroscopy approach could be applied to identify spectral alterations associated with the presence of insidious ovarian cancer. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)