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Quantifying nanoscale biochemical heterogeneity in human epithelial cancer cells using combined AFM and PTIR absorption nanoimaging
Author(s) -
Kennedy Eamonn,
AlMajmaie Rasoul,
AlRubeai Mohamed,
Zerulla Dominic,
Rice James H.
Publication year - 2015
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201300138
Subject(s) - atomic force microscopy , nanoscopic scale , nanotechnology , materials science , absorption (acoustics) , biophysics , biology , composite material
Subcellular chemical heterogeneity plays a key role in cell organization and function. However the biomechanics underlying the structure‐function relationship is governed by cell substructures which are poorly resolved using conventional chemical imaging methods. To date, advances in sub‐diffraction limited infrared (IR) nanoscopy have permitted intracellular chemical mapping. In this work we report how image analysis applied to a combination of IR absorption nanoimaging and topographic data permits quantification of chemical complexity at the nanoscale, enabling the analysis of biochemical heterogeneity in mammalian cancer cells on the scale of subcellular features. (© 2013 WILEY‐VCH Verlag GmbH &Co. KGaA, Weinheim)