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Raman spectroscopic evidence of tissue restructuring in heat‐induced tissue fusion
Author(s) -
Su Lei,
Cloyd Kristy L.,
Arya Shobhit,
Hedegaard Martin A. B.,
Steele Joseph A. M.,
Elson Daniel S.,
Stevens Molly M.,
Hanna George B.
Publication year - 2014
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201300099
Subject(s) - raman spectroscopy , fusion , characterization (materials science) , materials science , biophysics , biomedical engineering , chemistry , nanotechnology , optics , medicine , biology , philosophy , linguistics , physics
Heat‐induced tissue fusion via radio‐frequency (RF) energy has gained wide acceptance clinically and here we present the first optical‐Raman‐spectroscopy study on tissue fusion samples in vitro . This study provides direct insights into tissue constituent and structural changes on the molecular level, exposing spectroscopic evidence for the loss of distinct collagen fibre rich tissue layers as well as the denaturing and restructuring of collagen crosslinks post RF fusion. These findings open the door for more advanced optical feedback‐control methods and characterization during heat‐induced tissue fusion, which will lead to new clinical applications of this promising technology. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)