Label‐free evanescent microscopy for membrane nano‐tomography in living cells | Zendy

Bon Pierre | Zendy; Barroca Thomas | Zendy; LévèqueFort Sandrine | Zendy; Fort Emmanuel | Zendy

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Label‐free evanescent microscopy for membrane nano‐tomography in living cells

Author(s) -

Bon Pierre,

Barroca Thomas,

LévèqueFort Sandrine,

Fort Emmanuel

Publication year - 2014

Publication title -

journal of biophotonics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.877

H-Index - 66

eISSN - 1864-0648

pISSN - 1864-063X

DOI - 10.1002/jbio.201300095

Subject(s) - membrane , microscopy , microscope , tomography , live cell imaging , tracking (education) , nano , living cell , nanotechnology , materials science , optics , chemistry , biophysics , biological system , cell , physics , biology , biochemistry , psychology , pedagogy , composite material

We show that through‐the‐objective evanescent microscopy (epi‐EM) is a powerful technique to image membranes in living cells. Readily implementable on a standard inverted microscope, this technique enables full‐field and real‐time tracking of membrane processes without labeling and thus signal fading. In addition, we demonstrate that the membrane/interface distance can be retrieved with 10 nm precision using a multilayer Fresnel model. We apply this nano‐axial tomography of living cell membranes to retrieve quantitative information on membrane invagination dynamics. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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