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Effect of N‐ethylmaleimide, chymotrypsin, and H 2 O 2 on the viscoelasticity of human erythrocytes: Experimental measurement and theoretical analysis
Author(s) -
Chen YinQuan,
Chen ChihWei,
Ni YuLi,
Huang YuShan,
Lin Orson,
Chien Shu,
Sung Lanping Amy,
Chiou Arthur
Publication year - 2014
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201300081
Subject(s) - viscoelasticity , chemistry , viscosity , elasticity (physics) , dynamic modulus , biophysics , erythrocyte deformability , modulus , dynamic mechanical analysis , chymotrypsin , young's modulus , optical tweezers , red blood cell , thermodynamics , materials science , biochemistry , composite material , enzyme , organic chemistry , optics , trypsin , physics , biology , polymer
The physiological functions of erythrocytes depend critically on their morphology, deformability, and aggregation capability in response to external physical and chemical stimuli. The dynamic deformability can be described in terms of their viscoelasticity. We applied jumping optical tweezers to trap and stretch individual red blood cells (RBCs) to characterize its viscoelasticity in terms of the Young's modulus and viscosity by analyzing the experimental data of dynamic deformation using a 2‐parameter Kelvin solid model. The effects of three chemical agents ( N ‐ethylmaleimide, Chymotrypsin, and Hydrogen peroxide) on RBC's mechanical properties were studied by comparing the Young's modulus and viscosity of RBCs with and without these chemical treatments. Although the effects of each of these chemicals on the molecular structures of RBC may not be exclusive, based on the dominant effect of each chemical, we attempted to dissect the main contributions of different constituents of the RBC membrane to its viscosity and elasticity. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)