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Hyperspectral enhanced dark field microscopy for imaging blood cells
Author(s) -
Verebes Giulia Sacco,
Melchiorre Michele,
GarciaLeis Adianez,
Ferreri Carla,
Marzetti Carla,
Torreggiani Armida
Publication year - 2013
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201300067
Subject(s) - hyperspectral imaging , dark field microscopy , microscopy , materials science , artificial intelligence , pathology , computer science , medicine
Abstract In this work, a novel methodology based on hyperspectral imagery with enhanced Darkfield microscopy for probing and characterizing changes in blood cell components was tested. Two main categories of blood cells were analyzed, red and white blood cells. Unique spectral signatures of ordinary and most common deformed morphologies of red blood cells were identified. Moreover, examination of white blood cells allowed to characterize and differentiate active from inactive cells. The findings indicate the ability of this technique to detect changes in light scattering property of blood cells due to their morphological properties Since pathological states can alterate the discocyte shape, this preliminary, but promising application of the hyperspectral analysis to blood cells can be useful to evaluate significant correlations of blood cell spectral features in healthy and pathological conditions. The combination of the quali‐ and quantitative spectral signatures of hyperspectral imaging microscopy with the information of the subject health conditions may provide a new tool for clinical applications. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)