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Monte Carlo simulation of in vivo Raman spectral Measurements of human skin with a multi‐layered tissue optical model
Author(s) -
Wang Shuang,
Zhao Jianhua,
Lui Harvey,
He Qingli,
Bai Jintao,
Zeng Haishan
Publication year - 2014
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201300045
Subject(s) - raman spectroscopy , stratum corneum , human skin , raman scattering , monte carlo method , materials science , optics , in vivo , dermis , epidermis (zoology) , molecular physics , biomedical engineering , chemistry , pathology , physics , anatomy , medicine , statistics , mathematics , microbiology and biotechnology , biology , genetics
Raman photon generation inside human skin and escaping to skin surface were modeled in an eight‐layered skin optical model. Intrinsic Raman spectra of different skin layers were determined by microscopy measurements of excised skin tissue sections. Monte Carlo simulation was used to study the excitation light distribution and intrinsic Raman signal distortion caused by tissue reabsorption and scattering during in vivo measurements. The simulation results demonstrated how different skin layers contributed to the observed in vivo Raman spectrum. Using the strongest Raman peak at 1445 cm –1 as an example, the simulation suggested that the integrated contributions of the stratum corneum layer is 1.3%, the epidermis layer 28%, the dermis layer 70%, and the subcutaneous fat layer 1.1%. Reasonably good matching between the calculated spectrum and the measured in vivo Raman spectra was achieved, thus demonstrated great utility of our modeling method and approaches for help understanding the clinical measurements. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)