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Imaging the noncentrosymmetric structural organization of tendon with Interferometric Second Harmonic Generation microscopy
Author(s) -
Rivard Maxime,
Popov Konstantin,
Couture CharlesAndré,
Laliberté Mathieu,
BertrandGrenier Antony,
Martin François,
Pépin Henri,
Pfeffer Christian P.,
Brown Cameron,
Ramunno Lora,
Légaré François
Publication year - 2014
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201300036
Subject(s) - tendon , interferometry , collagen fibril , polarity (international relations) , second harmonic generation , microscopy , fibril , transverse plane , materials science , optics , harmonic , biophysics , chemistry , anatomy , physics , biology , acoustics , laser , biochemistry , cell
We report the imaging of tendon with Interferometric Second Harmonic Generation microscopy. We observe that the noncentrosymmetric structural organization can be maintained along the fibrillar axis over more than 150 μm, while in the transverse direction it is ∼1–15 μm. Those results are explained by modeling tendon as a heterogeneous distribution of noncentrosymmetric nano‐cylinders (collagen fibrils) oriented along the fibrillar axis. The preservation of the noncentrosymmetric structural organization over multiple tens of microns reveals that tendon is made of domains in which the ratio between fibrils with positive and negative polarity is unbalanced. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)