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Monitoring caffeine and resveratrol cutaneous permeation by confocal Raman microspectroscopy
Author(s) -
Tfaili Sana,
Josse Gwendal,
Angiboust JeanFrançois,
Manfait Michel,
Piot Olivier
Publication year - 2014
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201300011
Subject(s) - confocal , raman microspectroscopy , chemistry , raman spectroscopy , resveratrol , caffeine , permeation , biophysics , kinetics , human skin , diffusion , in vivo , molecule , biochemistry , organic chemistry , optics , membrane , genetics , quantum mechanics , medicine , physics , microbiology and biotechnology , biology , endocrinology , thermodynamics
Dynamic follow‐up of exogenous molecules permeation through the skin is one among many competing applications for confocal Raman microspectroscopy. Previous studies showed the feasibility of tracking actives through the skin; the next step should be recording in vivo kinetics. Thus, we conducted a study to evaluate the possibility of detecting low concentrations of caffeine and resveratrol solutions through the skin using confocal Raman microspectroscopy. After topical application of each active on the skin surface, Raman profiles were recorded over nine hours. The challenge was to pursuit these actives respecting the concentration used in some dermatological formulations. Molecules were successfully detected and kinetic profiles were registered over time. The heterogeneity of skin structure and the complexity of molecules diffusion were reflected through the kinetic results. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)