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M13 Virus based detection of bacterial infections in living hosts
Author(s) -
Bardhan Neelkanth M.,
Ghosh Debadyuti,
Belcher Angela M.
Publication year - 2014
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201300010
Subject(s) - pilus , microbiology and biotechnology , bacteria , virus , pathogenic bacteria , bacteriophage , bacterial conjugation , biology , staphylococcus aureus , virology , fluorescence , in vivo , escherichia coli , gene , genetics , physics , quantum mechanics
We report a first method for using M13 bacteriophage as a multifunctional scaffold for optically imaging bacterial infections in vivo . We demonstrate that M13 virus conjugated with hundreds of dye molecules (M13‐Dye) can target and distinguish pathogenic infections of F‐ pili expressing and F ‐negative strains of E. coli . Further, in order to tune this M13‐Dye complex suitable for targeting other strains of bacteria, we have used a 1‐step reaction for creating an anti‐ bacterial antibody ‐M13‐Dye probe. As an example, we show anti‐ S. aureus ‐M13‐Dye able to target and image infections of S. aureus in living hosts, with a 3.7× increase in fluorescence over background. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)