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Optical induction of muscle contraction at the tissue scale through intrinsic cellular amplifiers
Author(s) -
Yoon Jonghee,
Choi Myunghwan,
Ku Taeyun,
Choi Won Jong,
Choi Chulhee
Publication year - 2014
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200246
Subject(s) - contraction (grammar) , ryanodine receptor , endoplasmic reticulum , intracellular , biophysics , muscle contraction , myocyte , calcium in biology , chemistry , vascular smooth muscle , calcium , microbiology and biotechnology , biology , anatomy , endocrinology , smooth muscle , biochemistry , organic chemistry
The smooth muscle cell is the principal component responsible for involuntary control of visceral organs, including vascular tonicity, secretion, and sphincter regulation. It is known that the neurotransmitters released from nerve endings increase the intracellular Ca 2+ level in smooth muscle cells followed by muscle contraction. We herein report that femtosecond laser pulses focused on the diffraction‐limited volume can induce intracellular Ca 2+ increases in the irradiated smooth muscle cell without neurotransmitters, and locally increased intracellular Ca 2+ levels are amplified by calcium‐induced calcium‐releasing mechanisms through the ryanodine receptor, a Ca 2+ channel of the endoplasmic reticulum. The laser‐induced Ca 2+ increases propagate to adjacent cells through gap junctions. Thus, ultrashort‐pulsed lasers can induce smooth muscle contraction by controlling Ca 2+ , even with optical stimulation of the diffraction‐limited volume. This optical method, which leads to reversible and reproducible muscle contraction, can be used in research into muscle dynamics, neuromuscular disease treatment, and nanorobot control. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)