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Development of an integrated optical analyzer for characterization of growth dynamics of bacterial colonies
Author(s) -
Kim Huisung,
Bai Nan,
Bhunia Arun K.,
King Galen B.,
Hirleman E. Daniel,
Bae Euiwon
Publication year - 2013
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200224
Subject(s) - optical density , dynamics (music) , bacterial colony , bacterial growth , confocal , biological system , displacement (psychology) , optics , nanotechnology , materials science , biology , chemistry , bacteria , physics , acoustics , psychology , psychotherapist , genetics
In order to understand the biophysics behind collective behavior of a bacterial colony, a confocal displacement meter was used to measure the profiles of the bacterial colonies, together with a custom built optical density circuits. The system delivered essential information related to the quantitative growth dynamics (height, diameter, aspect ratio, optical density) of the bacterial colony. For example, the aspect ratio of S. aureus was approximately two times higher than that of E. coli O157 : H7, while the OD of S. aureus was approximately 1/3 higher than that of E. coli O157 : H7. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)