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Non‐linear optical microscopy of kidney tumours
Author(s) -
Galli Roberta,
Sablinskas Valdas,
Dasevicius Darius,
Laurinavicius Arvydas,
Jankevicius Feliksas,
Koch Edmund,
Steiner Gerald
Publication year - 2014
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200216
Subject(s) - pathology , microscopy , kidney , renal cell carcinoma , cancer , chemistry , medicine , biology
The unregulated cancer cell growth leads to strong alterations in morphology and composition of the tissue. The combination of coherent anti‐Stokes Raman scattering, two‐photon excited fluorescence and second harmonic generation enables a high resolution imaging with strong information on tissue composition and can then provide useful information for tumour diagnosis. Here we present the potential of multimodal non‐linear microscopy for imaging of renal tumours. Using cryosections of human oncocytoma and carcinoma, the method gave a detailed insight in cancer morphology and composition, enabling to discern between normal kidney tissue, tumour and necrosis. Several features significant for the diagnosis were clearly visualised without use of any staining. Translation of this method in clinical pathology will greatly improve speed and quality of the analyses. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)