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Measuring tumor cycling hypoxia and angiogenesis using a side‐firing fiber optic probe
Author(s) -
Yu Bing,
Shah Amy,
Wang Bingqing,
Rajaram Narasimhan,
Wang Quanli,
Ramanujam Nirmala,
Palmer Gregory M.,
Dewhirst Mark W.
Publication year - 2014
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200187
Subject(s) - hypoxia (environmental) , angiogenesis , biomedical engineering , cycling , optical fiber , materials science , medicine , chemistry , optics , cancer research , oxygen , physics , organic chemistry , archaeology , history
Hypoxia and angiogenesis can significantly influence the efficacy of cancer therapy and the behavior of surviving tumor cells. There is a growing demand for technologies to measure tumor hypoxia and angiogenesis temporally in vivo to enable advances in drug development and optimization. This paper reports the use of frequency‐domain photon migration with a side‐firing probe to quantify tumor oxygenation and hemoglobin concentrations in nude rats bearing human head/neck tumors administered with carbogen gas, cycling hypoxic gas or just room air. Significant increase (with carbogen gas breathing) or decrease (with hypoxic gas breathing) in tumor oxygenation was observed. The trend in tumor oxygenation during forced cycling hypoxia (CH) followed that of the blood oxygenation measured with a pulse oximeter. Natural CH was also observed in rats under room air. The studies demonstrated the potential of the technology for longitudinal monitoring of tumor CH during tumor growth or in response to therapy. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)