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Hyperspectral multimodal CARS microscopy in the fingerprint region
Author(s) -
Pegoraro Adrian F.,
Slepkov Aaron D.,
Ridsdale Andrew,
Moffatt Douglas J.,
Stolow Albert
Publication year - 2014
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200171
Subject(s) - hyperspectral imaging , microscopy , fingerprint (computing) , raman spectroscopy , raman scattering , optics , ultrashort pulse , materials science , chemical imaging , photonics , optoelectronics , computer science , physics , artificial intelligence , laser
A simple scheme for multimodal coherent anti‐Stokes Raman scattering (CARS) microscopy is based on the spectral focusing of ultrafast‐oscillator‐derived pump/probe light and synchronous photonic crystal fiber (PCF) fiber‐generated broadband Stokes light. To date, such schemes allowed rapid hyperspectral imaging throughout the CH/OH high frequency region (2700–4000 cm ‐1 ). Here we extend this approach to the middle (1640–3300 cm ‐1 ) and fingerprint regions (850–1800 cm ‐1 ) of the Raman spectrum. Our simple integrated approach to rapid hyperspectral CARS microscopy in the fingerprint region is demonstrated by applications to label‐free multimodal imaging of cellulose and bulk bone, including use of the phosphate resonance at 960 cm ‐1 . (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)