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Characterization of atherosclerotic plaque depositions by Raman and FTIR imaging
Author(s) -
Lattermann Annika,
Matthäus Christian,
Bergner Norbert,
Beleites Claudia,
Romeike Bernd F.,
Krafft Christoph,
Brehm Bernhard R.,
Popp Jürgen
Publication year - 2013
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200146
Subject(s) - raman spectroscopy , microscopy , fourier transform infrared spectroscopy , materials science , van gieson's stain , chemistry , infrared microscopy , biomedical engineering , pathology , h&e stain , staining , analytical chemistry (journal) , optics , medicine , chromatography , physics
Spectroscopy‐based imaging techniques can provide useful biochemical information about tissue samples. Here, we employ Raman and Fourier transform infrared (IR) imaging to characterize composition and constitution of atherosclerotic plaques of rabbits, fed with a high cholesterol diet. The results were compared with conventional light microscopy after staining with hematoxylin eosin, and elastica van Gieson. The spectral unmixing algorithm vertex component analysis was applied for data analysis and image reconstruction. IR microscopy allowed for differentiation between lipids and proteins in plaques of full aortic cross sections. Raman microscopy further discriminated cholesterol esters, cholesterol and triglycerides. FTIR and Raman images were recorded at a resolution near 20 micrometer per pixel for a large field of view. High resolution Raman images at 1 micrometer per pixel revealed structural details at selected regions of interest. The intima‐media and the lipid‐protein ratio were determined in five specimens for quantitation. These results correlate well with histopathology. The described method is a promising tool for easy and fast molecular imaging of atherosclerosis. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)