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Discrimination of single‐porin Escherichia ( E. ) coli mutants by ATR and transmission mode FTIR spectroscopy
Author(s) -
Saraiva Raúl G.,
Lopes João Almeida,
Machado Jorge,
Gameiro Paula,
Feio Maria J.
Publication year - 2014
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200131
Subject(s) - porin , fourier transform infrared spectroscopy , attenuated total reflection , spectroscopy , chemistry , mutant , escherichia coli , analytical chemistry (journal) , infrared spectroscopy , bacterial outer membrane , biology , biochemistry , chromatography , physics , optics , gene , organic chemistry , quantum mechanics
Vibrational spectroscopy has long been used in bacterial identification with different levels of taxonomic discrimination but its true potential for intra‐species differentiation remains poorly explored. Herein, both transmission Fourier‐transform infrared (FTIR) and attenuated total reflectance (ATR)‐FTIR spectroscopy are used to analyse E. coli strains that differ solely in their porin expression profile. In this previously unreported approach, the applicability of both FTIR‐spectroscopy techniques is compared with the same collection of unique strains. ATR‐FTIR spectroscopy proved to reliably distinguish between several E. coli porin mutants with an accuracy not replicated by FTIR in transmission mode (using previously optimized procedures). Further studies should allow the identification of the individual contribution of the single porin channel to the overall bacterial infrared spectrum and of molecular predictive patterns of porin alterations. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)