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Antimicrobial photodynamic therapy with RLP068 kills methicillin‐resistant Staphylococcus aureus and improves wound healing in a mouse model of infected skin abrasion PDT with RLP068/Cl in infected mouse skin abrasion
Author(s) -
Vecchio Daniela,
Dai Tianhong,
Huang Liyi,
Fantetti Lia,
Roncucci Gabrio,
Hamblin Michael R.
Publication year - 2013
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200121
Subject(s) - photodynamic therapy , staphylococcus aureus , wound healing , microbiology and biotechnology , antimicrobial , in vivo , bioluminescence , abrasion (mechanical) , medicine , bacteria , methicillin resistant staphylococcus aureus , chemistry , biology , surgery , materials science , biochemistry , genetics , organic chemistry , composite material
Photodynamic therapy (PDT) is an alternative treatment for infections that can kill drug resistant bacteria without damaging host‐tissue. In this study we used bioluminescent methicillin‐resistant Staphylococcus aureus , in a mouse skin abrasion model, to investigate the effect of PDT on bacterial inactivation and wound healing. RLP068/Cl, a tetracationic Zn(II)phthalocyanine derivative and toluidine blue (TBO) were used. The light‐dose response of PDT to kill bacteria in vivo and the possible recurrence in the days post‐treatment were monitored by real‐time bioluminescence imaging, and wound healing by digital photography. The results showed PDT with RLP068/Cl (but not TBO) was able to kill bacteria, to inhibit bacterial re‐growth after the treatment and to significantly accelerate the wound healing process (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)