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Diffuse reflectance spectroscopy in Barrett’s Esophagus: developing a large field‐of‐view screening method discriminating dysplasia from metaplasia
Author(s) -
Douplik Alexandre,
Zanati Simon,
Saiko Guennadi,
Streutker Catherine,
Loshchenov Maxim,
Adler Desmond,
Cho Sarah,
Chen Dean,
Cirocco Maria,
Marcon Norman,
Fengler John,
Wilson Brian C.
Publication year - 2014
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200114
Subject(s) - dysplasia , autofluorescence , esophagus , intestinal metaplasia , spectroscopy , endoscopy , diffuse reflectance infrared fourier transform , barrett's esophagus , metaplasia , medicine , pathology , optics , chemistry , radiology , physics , adenocarcinoma , cancer , fluorescence , biochemistry , quantum mechanics , photocatalysis , catalysis
We evaluated diffuse reflectance spectroscopy implemented as a small field‐of‐view technique for discrimination of dysplasia from metaplasia in Barrett’s esophagus as an adjuvant to autofluorescence endoscopy. Using linear discriminant analysis on 2579 spectra measured in 54 patients identified an optimum a 4‐wavelength classifier (at 485, 513, 598 and 629 nm). Sensitivity and specificity for a test data set were 0.67 and 0.85, respectively. Spectroscopic results show that this technique could be implemented in wide‐field imaging mode to improve the accuracy of existing endoscopy techniques for finding early pre‐malignant lesions in Barrett’s esophagus. Results show that the discrimination occurs likely due to redistribution of blood content in the tissue sensed by the optical probing with the wavelength‐dependent sampling depth. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)