Premium
Raman spectroscopy provides a powerful, rapid diagnostic tool for the detection of tuberculous meningitis in ex vivo cerebrospinal fluid samples
Author(s) -
Sathyavathi R.,
Dingari Narahara Chari,
Barman Ishan,
Prasad P. S. R.,
Prabhakar Subhashini,
Narayana Rao D.,
Dasari Ramachandra R.,
Undamatla Jayanthi
Publication year - 2013
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200110
Subject(s) - tuberculous meningitis , mycobacterium tuberculosis , ex vivo , cerebrospinal fluid , raman spectroscopy , gold standard (test) , tuberculosis , meningitis , medicine , pathology , microbiology and biotechnology , in vivo , biology , surgery , radiology , physics , optics
Abstract In this letter, we propose a novel method for diagnosis of tuberculous meningitis using Raman spectroscopy. The silicate Raman signature obtained from Mycobacterium tuberculosis positive cases enables specific and sensitive detection of tuberculous meningitis from acquired cerebrospinal fluid samples. The association of silicates with the tuberculosis mycobacterium is discussed. We envision that this new method will facilitate rapid diagnosis of tuberculous meningitis without application of exogenous reagents or dyes and can be aptly used as a complementary screening tool to the existing gold standard methods. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)