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Second harmonic generation microscopy as a powerful diagnostic imaging modality for human ovarian cancer
Author(s) -
Adur Javier,
Pelegati Vitor B.,
de Thomaz Andre A.,
Baratti Mariana O.,
Andrade Liliana A. L. A.,
Carvalho Hernandes F.,
BottcherLuiz Fátima,
Cesar Carlos Lenz
Publication year - 2014
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200108
Subject(s) - stroma , ovarian cancer , serous fluid , pathology , extracellular matrix , ovary , microscopy , modality (human–computer interaction) , medicine , biology , cancer , computer science , microbiology and biotechnology , artificial intelligence , immunohistochemistry
In this study we showed that second‐harmonic generation (SHG) microscopy combined with precise methods for images evaluation can be used to detect structural changes in the human ovarian stroma. Using a set of scoring methods (alignment of collagen fibers, anisotropy, and correlation), we found significant differences in the distribution and organization of collagen fibers in the stroma component of serous, mucinous, endometrioid and mixed ovarian tumors as compared with normal ovary tissue. This methodology was capable to differentiate between cancerous and healthy tissue, with clear cut distinction between normal, benign, borderline, and malignant tumors of serous type. Our results indicated that the combination of different image‐analysis approaches presented here represent a powerful tool to investigate collagen organization and extracellular matrix remodeling in ovarian tumors (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)