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Development and comparative assessment of Raman spectroscopic classification algorithms for lesion discrimination in stereotactic breast biopsies with microcalcifications
Author(s) -
Dingari Narahara Chari,
Barman Ishan,
Saha Anushree,
McGee Sasha,
Galindo Luis H.,
Liu Wendy,
Plecha Donna,
Klein Nina,
Dasari Ramachandra Rao,
Fitzmaurice Maryann
Publication year - 2013
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200098
Subject(s) - fibroadenoma , breast cancer , support vector machine , artificial intelligence , mammography , raman microscope , pattern recognition (psychology) , predictive value , raman spectroscopy , computer science , radiology , medicine , cancer , raman scattering , physics , optics
Microcalcifications are an early mammographic sign of breast cancer and a target for stereotactic breast needle biopsy. Here, we develop and compare different approaches for developing Raman classification algorithms to diagnose invasive and in situ breast cancer, fibrocystic change and fibroadenoma that can be associated with microcalcifications. In this study, Raman spectra were acquired from tissue cores obtained from fresh breast biopsies and analyzed using a constituent‐based breast model. Diagnostic algorithms based on the breast model fit coefficients were devised using logistic regression, C4.5 decision tree classification, k‐nearest neighbor ( k ‐NN) and support vector machine (SVM) analysis, and subjected to leave‐one‐out cross validation. The best performing algorithm was based on SVM analysis (with radial basis function), which yielded a positive predictive value of 100% and negative predictive value of 96% for cancer diagnosis. Importantly, these results demonstrate that Raman spectroscopy provides adequate diagnostic information for lesion discrimination even in the presence of microcalcifications, which to the best of our knowledge has not been previously reported. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)