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Measuring anisotropic cell motility on curved substrates
Author(s) -
Douglass Kyle M.,
Sparrow Nicklaus A.,
Bott Marga,
FernandezValle Cristina,
Dogariu Aristide
Publication year - 2013
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200089
Subject(s) - motility , curvature , radius of curvature , radius , laminin , cylinder , cell , biophysics , materials science , chemistry , biology , microbiology and biotechnology , mathematics , geometry , mean curvature , computer science , biochemistry , computer security , mean curvature flow
Schwann cell motility was observed on laminin‐coated quartz cylinders with different curvatures over an 18 hour period. A new analysis based on difference images helped to determine the minimal radius of curvature, 46 μm, which restricted motility along the cylinder axis. The migration speed, measured by calculating differences between successive images in the time series, ranged between 0.3 to 0.8 μm per minute and is similar to previously reported rates for Schwann cells. Difference images provide a rapid and simple method for the analysis of cell motility on large populations of cells. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)