Premium
Identification of carbapenem‐resistant Acinetobacter baumannii clones using infrared spectroscopy
Author(s) -
Sousa Clara,
Grosso Filipa,
MeirinhosSoares Luís,
Peixe Luísa,
Lopes João
Publication year - 2014
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200075
Subject(s) - acinetobacter baumannii , fourier transform infrared spectroscopy , typing , microbiology and biotechnology , infrared spectroscopy , biology , chemistry , bacteria , genetics , physics , pseudomonas aeruginosa , organic chemistry , quantum mechanics
In this work we assessed the discriminatory ability of Fourier‐transform Infrared Spectroscopy (FTIR) in 22 representative isolates from a collection of 318 carbapenem‐hydrolyzing class D β ‐lactamases (CHDL)‐producing Acinetobacter spp. (5 hospitals; 2001–2008) previously characterized by DNA‐based typing methods. FTIR spectra were acquired with a Bruker spectrometer and analyzed with support of several chemometric tools. The results showed that FTIR spectroscopy was able to distinguish the main CHDL‐producing Acinetobacter baumannii lineages causing infection in Portugal, the ST103 carrying bla OXA‐58 , ST98 carrying bla OXA‐24/40 and ST92 carrying bla OXA‐23 . Moreover, this study revealed distinctive phenotypic features of A. baumannii lineages causing infections that might justify different epidemic potential. Spectroscopy may arise as a low cost and easily to perform alternative for typing A. baumannii isolates. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)