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A quantitative bioapatite/collagen calibration method using Raman spectroscopy of bone
Author(s) -
Karampas I. A.,
Orkoula M. G.,
Kontoyannis C. G.
Publication year - 2013
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200053
Subject(s) - raman spectroscopy , calibration , hydroxyproline , analytical chemistry (journal) , chemistry , materials science , chromatography , physics , optics , biochemistry , quantum mechanics
Numerous calibration models were developed and tested for the quantitative analysis of collagen and bioapatite in bone using Raman spectroscopy. The ν 1 phosphate vibration at 960 cm –1 was used as indicator of the content of bioapatite while for collagen three markers were used: the C–H 2 band at 2940 cm –1 , the amide I band at 1667 cm –1 and the vibrations of proline and hydroxyproline at 855 and 878 cm –1 , respectively. Also a calibration model based on the PLS algorithm was developed, too. Validation of the derived calibration models indicated that the model that makes use of the height ratio of the peaks 960/(855+878) exhibits the best accuracy. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)