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Nanofluidic devices towards single DNA molecule sequence mapping
Author(s) -
Marie Rodolphe,
Kristensen Anders
Publication year - 2012
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200050
Subject(s) - optical mapping , dna , genomics , nanotechnology , sequence (biology) , genomic dna , computational biology , dna sequencing , nanofluidics , microfluidics , chemistry , genome , biology , materials science , gene , genetics , biochemistry
Nanofluidics enables the imaging of stretched single molecules with potential applications for single molecule sequence mapping. Lab‐on‐a‐chip devices for single cell trapping and lyzing, genomic DNA extraction from single cells, and optical mapping of genomic length DNA has been demonstrated separately. Yet the pursuit for applying DNA optical mapping to solve real genomics challenges is still to come. We review lab‐on‐a‐chip devices from literature that could be part of a complete system for the sequence mapping of single DNA molecules. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)