Quantitative analysis of the redox states of cytochromes in a living L929 (NCTC) cell by resonance Raman microspectroscopy | Zendy

Kakita Minoru | Zendy; Okuno Masanari | Zendy; Hamaguchi Hiroo | Zendy

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Quantitative analysis of the redox states of cytochromes in a living L929 (NCTC) cell by resonance Raman microspectroscopy

Author(s) -

Kakita Minoru,

Okuno Masanari,

Hamaguchi Hiroo

Publication year - 2013

Publication title -

journal of biophotonics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.877

H-Index - 66

eISSN - 1864-0648

pISSN - 1864-063X

DOI - 10.1002/jbio.201200042

Subject(s) - raman spectroscopy , redox , chemistry , resonance raman spectroscopy , cytochrome c , cytochrome , resonance (particle physics) , raman microspectroscopy , photochemistry , analytical chemistry (journal) , biochemistry , biophysics , inorganic chemistry , chromatography , mitochondrion , biology , enzyme , physics , particle physics , optics

Raman spectra and images of a living L929 (NCTC) cell have been measured with 532 nm excitation. Both reduced and oxidized forms of cytochromes b and c (cyt b and cyt c ) have been observed in situ without any pretreatment. The redox states of cyts b and c have been assessed quantitatively with a spectral analysis. It has been found that reduced cyt c is more abundant than oxidized cyt c , while oxidized cyt b is slightly more abundant than reduced cyt b in a living cell. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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