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Quantitative analysis of the redox states of cytochromes in a living L929 (NCTC) cell by resonance Raman microspectroscopy
Author(s) -
Kakita Minoru,
Okuno Masanari,
Hamaguchi Hiroo
Publication year - 2013
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200042
Subject(s) - raman spectroscopy , redox , chemistry , resonance raman spectroscopy , cytochrome c , cytochrome , resonance (particle physics) , raman microspectroscopy , photochemistry , analytical chemistry (journal) , biochemistry , biophysics , inorganic chemistry , chromatography , mitochondrion , biology , enzyme , physics , particle physics , optics
Raman spectra and images of a living L929 (NCTC) cell have been measured with 532 nm excitation. Both reduced and oxidized forms of cytochromes b and c (cyt b and cyt c ) have been observed in situ without any pretreatment. The redox states of cyts b and c have been assessed quantitatively with a spectral analysis. It has been found that reduced cyt c is more abundant than oxidized cyt c , while oxidized cyt b is slightly more abundant than reduced cyt b in a living cell. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)